Sexual isolation in bacteria

Isolation of Cellulolytic Bacteria

Isolation of Bacteria

Occurrence of antimicrobial resistant bacterial strains is a key issue for therapeutic outcome both in human and veterinary medicine. Moreover, when considering food animals the risk of their contribution to antibiotic resistance in humans has to be taken into account. Consequently surveys are needed to describe evolution of drug sensitivity in pathogens and indicator bacteria obtained from the...

Sexual conflict and reproductive isolation in flies.

Sexual conflict is predicted to generate more rapid reproductive isolation between larger populations. While there is some empirical support for this, the data are inconsistent and, additionally, there has been criticism of some of the evidence. Here we reanalyse two experimental-evolution datasets using an isolation index widely applied in the speciation literature. We find evidence for reprod...

Isolation of Gram-negative Bacteria

Three different areas -hospital, food-handling establishments and human dwellings, were surveyed for pathogenic gram-negative bacteria carried on the cuticles of cockroaches. Fifty species of bacteria were identified from all cockroaches. Escherichia coli, Klebsiella pneumoniae, Citrobacter freundii and Enterobacter cloacae were the most frequently found. Pathogenic and potentially pathogenic b...

Isolation of bacteria envelope proteins.

Proteomic analysis on cell envelope proteins from Gram-negative bacteria requires specific isolation techniques. We found that conventional extraction methods such as osmotic shock cause extracts to be heavily contaminated with soluble cytoplasmic proteins. These cytoplasmic protein contaminants constitute the major signal in proteomic analysis and can overwhelm the signals coming from genuine ...